| Details: |
There has been a surge of interest in recent years to design and fabricate various types of motile
micro/nanoparticles that can be maneuvered using chemical, optical, thermal, electrical or magnetic energy
sources. A collection of such motile particles can be used as a model system to study various active matter
phenomena, which can answer fundamental questions related to non-equilibrium statistical physics. Of all types
of actuation techniques, magnetic actuation deserves special mention owing to its non-invasive and non-chemical
nature. However, designing magnetically actuated active matter system is not a trivial task. In the first half of
my talk, I will discuss how to design a helical structured, magnetically actuated, active matter system.
In the second half of my talk, I will discuss how protein components of the nuclear envelope organise DNA
to increase its mechanical stability to withstand forces that would otherwise damage double-stranded DNA. We
use a bottom-up reconstitution approach coupled with optical tweezer experiments, theoretical physics and cell
biology to investigate how the chromatin cross-bridging factor BAF, the inner nuclear membrane protein LEM2
and DNA self-organize. Our data point to a mechanism where BAF-LEM2 organize DNA into regular loops and
further jointly compact into large fluid-like dna-protein co-condensates. As a result, DNA is mechanically
protected in couple of ways: Firstly, DNA respond to increasing external forces with increased compaction
forces, suggesting that the protein-bound DNA molecule is mechanically stabilized. Secondly, forces with the
potential to damage DNA percolate through the co-condensate, reducing the mechanical stress on individual
DNA strands. Our study quantitatively describes a conserved and previously unknown molecular mechanism by
which cells use the nuclear envelope proteins as a self-assembling buffer against spontaneously occurring
mechanical stress to protect DNA from damage, suggesting a first preventive mechanism that adds to the cell's
ability to repair DNA damage or responsively change chromatin properties. |